Release of extracellular enzymes from Bacillus amyloliquefaciens

Release of extracellular enzymes from Bacillus amyloliquefaciens.

Washed-cell suspensions of Bacillus amyloliquefaciens secrete significant amounts of the extracellular enzymes alpha-amylase and protease for about 15 min in the almost complete absence of protein synthesis. This apparently represents release of preformed enzyme en route to secretion. The release was independent of energy but was affected by temperature. Pulse-labeling experiments showed that n...

Complementation of peptides of barnase, extracellular ribonuclease of Bacillus amyloliquefaciens.

Recovery of ribonuclease activity by complementation of peptides of barnase is reported. Activity is restored to barnase-(1-102), which lacks eight amino acids from its COOH terminus, by combination with peptides-(88-110), -(95-110), or -(99-108), and also with succinylated peptide-(88-110). The dissociation constants are about 8 X 10(-6) M for the first two combinations and little, if any, gre...

Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains

The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28oC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40oC and pH 4.0, resp...

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

Extracellular Xylanopectinolytic Enzymes by Bacillus subtilis ADI1 from EFB's Compost

Microbial xylanase and pectinase are two extremely valuable enzymes, which have captivated much attention. This can be seen from the increased demand for these enzymes by many industrial sectors. This study investigates the isolation and screening of extracellular xylanopectinolytic enzymes-producing bacteria in a submerged fermentation (SmF). Samples are collected from the compost of empty fru...